The hepatic and serum cholinesterase activities were studied in cholestasis induced by common bile duct(CBD) ligation and chronic ethanol intoxication, and in cholestasis after acute ethanol intoxication to establish the biochemical backgroud of
alcoholic hazard in hepatobiliary disease.
Exclusively, when CBD was ligate after chronic ethanol intoxication, the activities of hepatic mitochondrial and microsomal cholinesterase decreased more significantly than that of CBD was ligated only.
On the other hand, when CBD was ligated after chronic ethanol intoxication, the values of Vmax of the hepatic mitochondrial and microsomal cholinesterase decreased more significantly than that of CBD was ligated only. However, the values of Km of
above
hepatic cholinesterases did not change.
Viewed form above results, the hepatic mitochondrial and microsomal cholinesterase seem to be the enzymes which decrease activities in chronic ethanol intoxication with cholestasis more than in cholestasis, and the cause of the decrease are
suggested to
be decreased biosynthesis.
The results will be the data supporting that alcoholic drink is enzymologically harmful in hepatobiliary disease.
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